正交优化葛根黄酮提取工艺

范文编号:HG144  范文字数:11017,页数:26

 摘 要：对葛根黄酮提取工艺进行优化，得出较佳提取条件，可应用于葛根黄酮的工业化提取。实验以葛根黄酮提取率为指标，对葛根黄酮提取过程中的溶剂乙醇质量分数、提取时间、固液比、提取温度、pH、提取次数、重复性以及回收溶剂的再利用等因素进行了考察，最后用正交法进行优化。实验结果表明：葛根黄酮的初步提取工艺为溶剂乙醇质量分数为70%，提取时间为120min，固液比为1:10，提取温度为70℃，pH=5，提取次数1次；浸提操作重复性好，溶剂回收再利用可行并且溶剂的回收率高达94.3%。通过正交设计得出较佳条件为：乙醇质量分数为50%，提取时间为120min，固液比为1:12，提取温度为80℃。
 关键词：葛根黄酮；提取工艺；提取率；溶剂回收；正交设计

Abstract: The optimization experiment of puerarin flavonoids was carried out and the optimal extraction condition was determined, and accordingly to be used in industrialization extracting of puerarin flavonoids. In the experiment Puerarin flavonoids yield was taken as an index, mass fraction of ethanol, extraction time, solid-liquid ratio, extraction temperature, solution pH, extraction times, repeatability, recovery and reuse of solvent in the extraction process were studied, finally the experiment was optimized by orthogonal. The results showed that the mass fraction of ethanol was 70%, the extraction time was 120min, the solid-liquid ratio was 1:10, the extraction temperature was 70℃, the solution pH was 5, the extraction times was 1; repeatability for extraction operation was good, recycling solvents was feasible and the solvent recovery was up to 94.3%. The better conditions were obtained by orthogonal design as follows: the mass fraction of ethanol was 50%, the extraction time was 120min, the solid-liquid ratio was 1:12, the extraction temperature was 80℃.
 Keywords: Puerarin Flavonoids; Extraction process; Yield; Solvent recovery; Orthogonal design

 
目  录
中文摘要 I
英文摘要 II
目录 III
1．绪论 1
1.1  葛根的种类及分布 1
1.2  葛根的化学成分 1
1.3  葛根的利用 2
1.3.3  葛根的保健作用 3
1.4  葛根黄酮的提取方法 4
1.5  葛根黄酮的检测方法 4
1.6  葛根的发展前景 5
2．实验部分 8
2.1  实验原料、试剂与仪器 8
2.2  提取操作及分析过程 8
3．实验结果与讨论 9
3.1  标准曲线的绘制 9
3.2  单因素考察 10
3.3  正交设计 15
3.4  正交结果与分析 15
4．总结与展望 19
致谢 20
参考文献 21

 《正交优化葛根黄酮提取工艺》WORD格式全文下载链接