一株源于厌氧除磷反应器ＮＬ菌的鉴定及活性研究

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摘要： 使用从集中水池的泥沙作为接种物，磷酸盐脱氧磷酸盐入PH3的还原剂有机体在一台连续的被搅动的反应器被开化了。 功能细菌通过传统和现代隔离和证明方法被辨认了。 分类学位置被查明了根据配置的调查、生理和生物化学的物产和16S rDNA分析。 使用绝氧反应器在绝氧情况下，在细菌的基体退化的活动被调查了，表示， NL是一异养的dephosphorization细菌在绝氧情况下。 16S rDNA分析表明张力有98.2%与绿浓杆菌的同源。 它的最佳的碳源是葡萄糖和钠醋酸盐，并且最佳的氮气来源是氨盐基chloride、nitrate和胨。 然而，纤维素对碳是不合适的。 最佳的最初PH值是6.5～7.0，最佳温度是30～35 ℃。 当温度在4 ℃或在35 ℃之上，微生物的活动减少了。 有机磷和钙的加法有对磷撤除的分明影响，但是还原剂硫化物的加法有没有对磷撤除的明显的作用。 关键词： 绝氧dephosphorization; 绝氧dephosphorization细菌; 磷酸盐deoxidization; 膦

Abstract: Using the silt from concentrate pool as an inoculum, phosphate reducer organisms which deoxidize phosphate into PH3 were cultured in a continuous stirred reactor. The functional bacterium was identified through traditional and modern isolation and identification methods. The taxonomic position was ascertained based on the investigation of configuration, physiological and biochemical properties, and 16S rDNA analysis. The activity in substrate degradation of the bacterium was investigated using the anaerobic reactor under anaerobic condition, which showed that NL is a heterotrophic dephosphorization bacteria under anaerobic condition. The 16S rDNA analysis indicated the strain had a 98.2% of homology with Pseudomonas  aeruginosa. Its best carbon source is glucose and sodium acetate, and the best nitrogen source is ammonium chloride、nitrate and peptone. However, cellulose is unfit for carbon. The best initial pH value is 6.5～7.0, the optimum temperature is 30～35 ℃. The activity of microorganism decreased when the temperature was at 4 ℃ or above 35 ℃. The addition of organic phosphorus and calcium had distinct influence on the phosphorus removal, but the addition of reducer-sulfide had not apparent effect on the phosphorus removal.
Keywords: anaerobic dephosphorization; anaerobic dephosphorization bacteria; phosphate deoxidization; phosphine

目录
目录................................................................................ 1
1 前言...............................................................................1
2 材料与方法 ........................................................................3
2.1 使用仪器及设备...................................................................3
2.2 厌氧培养反应装置.................................................................3
2.2.1 厌氧除磷功能菌培养的混合连续流反应装置（见图1） ...............................3
2.2.2 厌氧除磷功能菌株的活性试验的反应瓶（见图3）....................................4
2.3 供试材料 ........................................................................5
2.3.1 供试种泥 ......................................................................5
2.3.2 培养基和基础培养液 ............................................................5
2.4 试验方法与步骤...................................................................5
2.4.1 厌氧除磷功能菌的培养...........................................................5
2.4.2 厌氧除磷功能菌株的分离、纯化与保存.............................................6
2.4.3 显微形态观察 ..................................................................6
2.4.4 厌氧除磷功能菌株的生理生化指标试验[13-15]......................................6
2.4.5 DNA提取、PCR扩增、基因序列比对及进化树构建 ....................................6
2.4.6 厌氧除磷功能菌株的活性试验.....................................................6
2.5 指标分析方法 ....................................................................7
2.5.1 总磷、磷酸盐采用钼酸铵分光光度法测定[21].......................................7
2.5.2 吸收液磷化氢测定...............................................................8
3 结果与讨论.........................................................................8
3.1 NL菌株的形态观察及生理生化指标...................................................8
3.2 NL菌株的16S rDNA基因的序列、系统发育分析 ........................................9
3.3 NL菌株的活性试验 ...............................................................10
3.3.1 营养条件对总磷去除及PH3生成的影响.............................................10
3.3.2 环境条件总磷去除及PH3生成的变化过程...........................................16
4 结论 .............................................................................20
参 考 文 献 ........................................................................21
附    录 ...........................................................................24
致    谢 ...........................................................................27

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