锁阳多糖的提取方法研究

范文编号:SW193  范文字数:11717,页数:27

摘要：锁阳（Cynomorium songaricum Rupr）是锁阳科锁阳属的单科单属单种植物,其肉质茎是我国的一种传统中药材，具有多种功效成分及营养成分。近些年关于锁阳的研究主要集中在资源调查、生物学特性、化学成分及药理等方面，但提取工艺研究却很少有报道。本文通过对锁阳多糖提取率的单因素试验和正交试验，得到了一个锁阳多糖的最佳提取工艺.结果显示在物料比为20：1，浸提时间为1h，提取温度80℃时锁阳多糖含量最高。同时对锁阳多糖提取液的脱蛋白方法进行了研究，以蛋白脱除率及多糖损失率为评价指标，对几种常见的脱蛋白方法进行了比较，确定了锁阳多糖的最佳脱蛋白方法为酶与Sevag结合法,从而为锁阳的活性成分和功效开发以及精深加工提供了理论基础。

Abstract：Cynomorium songaricum Rupr is an Cynomorium Cynomorium Branch alone in the Billing Division single plant, its succulent stems with a variety of effective composition and nutrient content is a traditional Chinese herbal medicine. In recent research , people paid main attention to the resource investigation, biology, chemistry and pharmacology, and so on. But research on  extraction technology have few report. This experiments based on the extraction rate of Cynomorium polysaccharide was optimized single factor and orthogonal design. The result showed that the optimum conditions for the extraction process as follows：the ratio of material to solution is 1：20 and the extraction at 80℃ for 1h．Meanwhile ,the methods of deproteinization from Cynomorium songaricum Rupr polysaccharide had been researched. We compared the several deproteinization method by the evaluation of their polysaccharide loss rate and deproteinization rate and eventually we identified the best method for deproteinization was enzyme and Sevag method. The basical research are hoped to provide a theoretical foundation for the active ingredient and effectiveness of development and intensive processing.
关键词：锁阳；多糖提取；脱蛋白

Key words：Cynomorium songaricum Rupr；extraction process of polysaccharides；deproteinization

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  锁阳简介 1
 1.1.1  锁阳的生境及资源分布 1
 1.1.2  锁阳的生物学特性 1
 1.1.3  锁阳的有效成分 2
 1.2  锁阳的综合开发研究现状及存在问题 3
 1.2.1  锁阳产品研发的现状 3
 1.2.2锁阳产品研发存在的问题 3
 1.3锁阳产品的市场需求和开发前景 4
2. 实验部分 5
 2.1  实验材料 5
 2.2  实验试剂 
 2.3  实验仪器 
 2.4  实验方法 
 2.4.1  锁阳多糖提取方法研究 
  2.4.2  锁阳多糖提取液脱蛋白方法的研究 
 3. 结果与讨论 
 3.1  锁阳多糖提取方法研究 
 3.1.1  总糖标准曲线 
 3.1.2  锁阳多糖提取率单因素试验 
  3.1.3  锁阳中多糖提取条件的正交试验 
 3.2  锁阳多糖提取液脱除蛋白质方法的选择 
 3.2.1  蛋白质标准曲线 
 3.2.2  不同浓度的三氯乙酸对锁阳多糖提取液的脱蛋白影响 
 3.2.3  不同次数的SEVAG法对锁阳多糖提取液的脱蛋白影响 
 3.2.4  不同的pH条件对锁阳多糖提取液的脱蛋白影响 
 3.2.5  酶法与不同次数的Sevag法对锁阳多糖提取液的脱蛋白影响 
 3.2.6  几种不同的脱蛋白方法对锁阳多糖提取液的脱蛋白影响 
4．总结与展望 
 4.1  总结 
 4.2  展望 
致谢 
参考文献 
附录 

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