水产中氯霉素残留分析

范文编号:SP101  范文字数:5082,页数:17  附任务书，开题报告，文献综述，外文翻译
摘 要：氯霉素（Chloramphenicol,CAP）是一种有效的广谱抗菌素，但同时对人体存在严重的毒副作用。本文采用固相萃取(SPE)前处理净化技术,用乙酸乙酯提取，正己烷脱脂，C18小柱净化，高效液相色谱-串联质谱(LC-MS/MS)法测定鱼中的氯霉素残留含量，采用内标法，以氯霉素峰面积/氯霉素-D5面积进行定量，以321.0/256.9作为定量离子对。氯霉素的检测线性范围均为0.05μg/kg～1.2μg/kg，回收率范围为70%～120%，321.0/256.9的标准曲线相关系数的最低值为0.99。液质联用法具有很高的灵敏度和稳定性, 是 FDA推荐的确证方法,液质联用法将成为国内首选的确证方法

关键词：氯霉素；液相色谱-串联质谱；同位素内标定量

Abstract: Chloramphenicol (Chloramphenicol, CAP) is an effective broad-spectrum antibiotics, but also on human existence of serious side effects. In this paper, solid-phase extraction (SPE) pre-treatment purification technology, extracted with ethyl acetate, n-hexane skim, C18 column purification, high-performance liquid chromatography - tandem mass spectrometry (LC-MS/MS) Determination of fish Chloramphenicol residues in, the standard method used to chloramphenicol peak area / chloramphenicol-D5 area of quantitative, 321.0/256.9 as a quantitative-right. Chloramphenicol linear range of detection are 0.05 μ g / kg ~ 1.2 μ g / kg, recovery rate of 70% to 120%, 321.0/256.9 standards of the lowest correlation coefficient of 0.99. Usage of MS have high sensitivity and stability, is recommended by the FDA confirmed methods, the use of quality will become the preferred method confirmed.

Keywords：Chloramphenicol; liquid chromatography - tandem mass spectrometry; isotope internal standard quantitative

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  理化性质 1
 1.2  抗菌作用 1 
 1.3  临床作用 2
 1.4  体内过程及对机体的危害 2
2. 实验部分 3
 2.1  仪器和设备 3
 2.2  试剂与溶液 4
 2.3  试验方法 5
 2.3.1 试样制备   5
     2.3.2 分析步骤   5
3. 结果与讨论 8
 3.1   内标法定量 8
 3.2  方法的线性关系和方法的测定低限 8
 3.3  精密度和回收率 9
 3.4  检测限、测定低限、线性范围、回收率范围和精密度 13
 3.5  色谱条件的优化 14
4．总结 14
致谢 15
参考文献 16

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