α-淀粉酶固定化的研究

范文编号:SW014  范文字数:8327,页数:20

摘 要：酶作为一种生物催化剂,因其具有高选择性、催化反应条件温和、无污染等特点,广泛应用于食品加工、医药和精细化工等行业，随着人类对环保的日益关注,酶的应用会更受关注。但自由酶的稳定性差、易失活、不能重复使用,纯化困难等缺点，使其难以在工业中更为广泛的应用。在此条件下,固定化酶的概念和技术得以提出和发展,此外，如何利用天然高分子载体,对其改性,或利用超临界技术、纳米技术、膜技术等来固定酶,也成为酶工程研究的热点。
 用聚苯乙烯阴离子交换树脂作载体，分别采用吸附法和吸附交联法将α—淀粉酶固定化，研究了固定化条件。结果表明，最佳固定化条件为：吸附温度6～9℃，pH值6.8，吸附时间16小时，交联温度6～9℃，交联时间8小时，戊二醛浓度4%。吸附交联法所得固定化α-淀粉酶稳定性较好，克服了传统吸附法的缺点，提高了固定化酶的酸碱稳定性、热稳定性、操作稳定性。

关键词：聚苯乙烯阴离子交换树脂；固定化；α-淀粉酶

Investigation of theα-amylase immobilization
Abstract: Enzyme, as a biological catalyst, is widely used in food processing, pharmaceuticals and fine chemical industries for its high selectivity, mild reaction, non-pollutaion and other good characteristics. With people's increasing attention to the environmental protection, the application of enzyme will be more concerned. However, there are many shortcomings of free enzyme, such as poor stability, easy deactivation, non-reuse, difficultly purification and so on. It is more difficult in wide range of industrial applications.. Under these conditions, the concepts and techniques of immobilization of enzyme are mentioned and developed. In addition, use of  natural polymer carrier and its modification, or use of some advanced technology, such as supercritical technology, nanometer technology, membrane technology to immobilize enzyme have become interesting topics on enzymatic engineering.
α-amylase was immobilized on polystyrene anion-exchange resin as support by two means, one is adsorption the other is adsorption and cross-linking. The conditions of immobilization were investigated. The optimum conditions for the immobilization were as follows: α-amylase was immobilized on polystyrene anion-exchange resin for 16h in 6~9℃ and pH 6.8,then cross-linked with 4% glutaraldehyde for 8h in 6~9℃. The stability of immobilized α-amylase is better in cross-linking, to overcome the traditional adsorption of shortcomings, and increase pH stability, thermal stability and operation stability of the immobilized α-amylase.

Key word: polystyrene anion-exchange resin；immobilization；α-amylase

目  录
中文摘要 I
英文摘要 II
目录 III
1 绪论 1
1.1固定化酶 1
1.2酶的固定化方法及其优缺点 2
1.3固定化酶载体的种类 4
1.4本文研究内容 5
2 实验部分 6
2.1材料 6
2.2方法 6
3 结果与讨论 8
3.1吸附法固定化条件的优选 8
3.2吸附交联法固定化条件的优选 9
3.3吸附法与吸附交联法的比较 11
4 总结 13
致谢 14
参考文献 15
附录A 吸光度与测试α-淀粉酶浓度对照表 16

 《α-淀粉酶固定化的研究》WORD格式全文下载链接