紫外诱变选育高产菌株的初步研究

范文编号:SW165  范文字数:9439,页数:27

摘 要：番茄红素是类胡萝卜素的一种,并具有较强的抗氧化作用,在食品、保健品、化妆品以及医药领域具有重要用途。利用三孢布拉氏霉(Blakeslea trispora)发酵生产番茄红素最有应用前景。本研究是利用含有洛伐他汀(lovastatin)和脱氧胆酸钠(SDC)的平板实现了对高产菌株的选育条件的最适浓度的确定，主要通过在固体培养基中添加不同浓度的脱氧胆酸钠和洛伐他汀，实验结果表明了三孢布拉氏霉在洛伐他汀(lovastatin)和脱氧胆酸钠(SDC)浓度分别在0.05﹪和0.03﹪的菌落局部生长效果最佳，而对孢子进行活化进行研究，实验结果表明4h培养时间为最佳时间，对于紫外光对三孢布拉氏霉菌进行照射处理致死率的确定，实验结果表明紫外照射7min的致死率为最适剂量，对诱变三孢布拉氏霉菌进行了初步研究。
关键词：番茄红素；紫外诱变；三孢布拉氏霉菌

Abstract: Lycopene is a carotenoid with strong antioxidant in food, health products, cosmetics, as well as an important field of medicine use. The use of three Blakeslea mycophenolate (Blakeslea trispora) Fermentation production of lycopene most applications. This study is the use of containing lovastatin (lovastatin) and sodium deoxycholate (SDC) to achieve the flat-panel high-yield strains of the selection conditions to determine the optimal concentration, primarily by adding the solid medium in different concentrations of deoxy - sodium cholate and lovastatin, experimental results show that three Blakeslea mycophenolate in lovastatin (lovastatin) and sodium deoxycholate (SDC) concentration of 0.05% and 0.03% growth of the colony the best local , and activation of the spores to carry out research, experimental results show that the 4h time for the best time to train for the three UV Blakeslea irradiated mold to determine the death rate, experimental results show that UV irradiation of the death rate of 7min the optimal dose for mutation three Blakeslea conducted a preliminary study of fungi.
Keywords：Lycopene ;Lovastatin;Blakeslea trispora

目录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  番茄红素性质 1
 1.2  番茄红素的生产方法 3
 1.3  本课题研究的内容 5
2. 实验部分 6
 2.1 试验材料 6
 2.2  试验设计及方法 7
3. 结果与讨论 9
 3.1   孢子最佳活化时间的确定 9
 3.2  不同脱氧胆酸钠浓度的确定   10
 3.3  不同洛伐他汀浓度的确定 12
 3.4  紫外诱变最佳时间的确定 14
 3.5  对紫外诱变后的筛选结果 18
4．总结与展望 20
致谢 21
参考文献 22

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