范文编号:SP065 范文字数:9850,页数:22 摘要:葡萄糖苷具有良好的功效,价格便宜,来源广,是一种较为理想的天然着色剂。本范文中用不同浓度的乙醇浓度进行超声波萃取,用吸光度来评价萃取含量的多少。在提取过程中的单实验因素中,通过改变乙醇的浓度、萃取的温度、萃取的时间以及萃取的料液比评定萃取效果,通过正交试验验证得出最佳组合。通过实验得出最佳的提取条件为:80%乙醇---HCl、R=1:12、T=50℃、t=40min。用邻苯三酚溶液、Fenton试剂、DPPH来测定葡萄糖苷的抗氧化能力。通过改变样品的浓度来得出清除率,由实验结果得出:邻苯三酚清除OH自由基的清除率为55%以上,Fenton试剂清除O2-自由基的能力的清除率为70%以上;DPPH清除自由基的清除率为70%以上。 关键词:葡萄糖苷 提取 邻苯三酚 Fenton DPPH Abstract:Glucoside has a good effect, cheap sources is broad, is an ideal natural coloring agent.In this paper, using different concentrations of ethanol concentration of ultrasonic extraction, extraction with absorbance to evaluate the amount of content.In the extraction process in a single experiment factors, by changing the ethanol concentration, extraction temperature, extraction time and extraction of the solid-liquid ratio assessment of extraction efficiency, obtained through the best combination of orthogonal test verification.Through experiments that the optimal extraction conditions were: 80% ethanol --- HCl, R = 1:12, T = 50 ℃, t = 40min.With pyrogallol solution, Fenton reagent, DPPH to measure the antioxidant capacity of grape glucosides.By changing the concentration of the sample to derive clearance rate, removal efficiency obtained by the experimental results have been more than 50%. 中文摘要…………………………………………………………………………… Ⅰ
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