|
范文编号:SW157 范文字数:12590,页数:30 摘 要：考查了马克斯克鲁维酵母 AS2.1440 在有机溶剂/水两相系统中生物转化L-苯丙氨酸生产 β-苯乙醇的过程。在单因素试验的基础上，用Plackett-Burman 设计、最陡爬坡试验结合响应面分析法对影响β-苯乙醇的因素进行了研究，确定了最佳发酵条件为：两相比例控制在油酸/水=2 : 3为宜；装液量为64.46 mL/150mL三角瓶，培养基初始pH控制在5.0，温度32.99℃，转速160 r/min下转化48 h，β-苯乙醇的产量可达1533±11 mg/L。与传统水相相比，油酸-水两相系统中Kluyveromyces marxianus AS2.1440 转化 L-苯丙氨酸生产 β-苯乙醇的产量提高了 102.91%。 关键词：两相体系；β-苯乙醇；响应面方法； AS2.1440 Abstract: Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 was performed in an aqueous/organic solvent biphasic system. On the basis of single-factor tests, factors affecting Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 were evaluated by Plackett-Burman design, the path of steepest ascent and response surface methodology. The optimal bioconversion conditions are as follows: the concentration of L-phenylalanine was 4 g/L, the optimum values of volume ratio of organic phase to aqueous phase, reaction temperature, pH value and liquid volume were 2 : 3, 32.99℃, 5.0, and 64.46mL/250mL respectively. Under above optimum conditions, the content of β-phenylethylalcohol reached1533±11g/L48 h later, which increased by about 102.91% than that in monophasic aqueous system. eywords：biphasic system; β-phenylethylalcohol; Response Surface Methodology; Kluyveromyces marxianus AS2.1440 目 录
|
| 上一篇：嘌呤啤酒酵母选育 | 下一篇：羊肚菌菌核形成影响因素 |
| 点击查看关于 响应 优化 体系 发酵 生产 天然 香料 乙醇 的相关范文题目 | 【返回顶部】 |